Applied biosystems stepone and steponeplus realtime pcr systems remarkably simple systems. Applied biosystems stepone steponeplus realtime pcr. Specifically designed with a user friendly, yet powerful interface, they are ideal for both new and experienced realtime pcr. Which version of the applied biosystems 7500 realtime pcr. The applied biosystems analysis software is a secure web application for analysis of data that are generated on thermo fisher scientific realtime pcr instruments. The applied biosystems analysis software can import and analyze experiment files eds and sds that are generated by various thermo fisher scientific realtime qpcr instruments.
Realtime pcr data analysis thermo fisher scientific us. The applied biosystems stepone and steponeplus realtime pcr systems offer intuitive software, fast pcfree or networked operation. Applied biosystems steponeplus realtime pcr system. These 96 well realtime pcr systems offers four color optical recording allowing for faster measurement. Applied biosystem 7500 fast real time pcr system andbio. Applied biosystems 7500 and 7500 fast software version 2.
Applied biosystems stepone and steponeplus realtime pcr systems getting started guide for 23 presenceabsence experiments notes. Applied biosystems quantstudio 5 realtime pcr system, 96. Applied biosystems 7500 realtime pcr system thermo fisher. The applied biosystems analysis software can import and analyze data generated by any of the supported instruments listed in the following table. Applied biosystems relative quantitation analysis module.
Field application specialist doug rains offers advices for fixing common software setup mistakes when performing realtime pcr. Robust and reliable applied biosystems thermal cyclers and pcr enzymes and reagents help you achieve success for virtually any application. Applied biosystems 7500 fast and 7500 realtime pcr. The applied biosystems 7500 rt pcr machine operates using a 96 well plate, a reaction volume range of 20100ul with a dynamic range of 9 logs, a block ramp rate of. Applied biosystems pioneered the development of realtime pcr in 1995, enabling highthroughput, quantitative gene expression. Compatible realtime pcr system data the applied biosystems analysis software can. View online or download applied biosystems 7500 installation and maintenance manual, getting started, getting started manual. The calibrate function is also available in the control console. Applied biosystems 7500 fast realtime pcr system thermo.
The standard genotyping experiment method was used on the applied biosystems quantstudio 5 realtime pcr system. The abi 7500 is the predecessor of the abi 5700, made as the lower throughput version of the abi 7900ht. Notes applied biosystems 75007500 fast realtime pcr system getting started guide for standard curve experiments. Prior to downloading any software to a computer used for instrument controloperation, please consult. The software provides projectbased analysis of realtime and endpoint data for various quantitative and qualitative pcr applications. For further information contact the director of licensing, applied biosystems, 850 lincoln. The applied biosystems 7500 realtime pcr system is a 3rd generation of thermocycling technology that allows quanitation of product generation in real time. Help from within applied biosystems 75007500 fast realtime pcr software v2. Each experiment file is a virtual representation of a.
Prior to downloading any software to a computer used for instrument controloperation, please consult with your service and support representative. The overall size and footprint of the abi 7500 was made more compact. This system is a sophisticated platform for users who require extended capabilities and maximum versatility. Applied biosystems 75007500 fast realtime pcr system getting started guide for genotyping experiments v preface how to use this guide about the system documentation the guides listed below are shipped with the applied biosystems 75007500 fast realtime pcr system 75007500 fast system. With either 3 or 6 independent temperature zones, the applied biosystems veriflex blocks within the quantstudio pro instruments provide flexibility to run multiple. Steponeplus realtime pcr systems getting started guide for genotyping experiments explains how to perform experiments on the stepone and steponeplus systems. Real time pcr model applied biosystems 7500 now on sale at the lab world group. Disclaimer the text above is not a recommendation to uninstall realtime pcr software v2.
Applied biosystems quantstudio 1 realtime pcr systems. How to use stepone plus qpcr machine by applied biosystems. Applied biosystems 7500 fast dx realtime pcr instrument. The hrm software is available on the 7500 fast realtime pcr system, which delivers precise results in a standard 96well format.
Applied biosystems 7500 realtime pcr system biocompare. The steponeplus realtime pcr system is a 96well realtime pcr instrument perfect for both firsttime and experienced users. Applied biosystems 7500 getting started pdf download. Used abi stepone qpcr on sale at the lab world group. Applied biosystems realtime pcr instruments thermo. The applied biosystems 730075007500 fast realtime pcr system site preparation guide provides the information you need to fully prepare your site for the arrival and installation of the applied biosystems 730075007500 fast real. Specially designed fast block ensures thermal uniformity at top speeds. Applied biosystems qpcr analysis modules within the thermo fisher cloud are web browserbased software tools that are flexible, fast and easy to use. The applied biosystems 7500 fast dx realtime pcr instrument with sds software is a realtime nucleic acid amplification and fivecolor fluorescence detection system available for in vitro diagnostic use.
The quantstudio 5 realtime pcr system is compact in form, factory calibrated and is delivered in userfriendly packaging to enable fast and simple installation for immediate use. Applied biosystems 7500 fast realtime pcr system, desktop. The software versions listed in the table represent only those tested for use with the applied biosystems software. The 7500 realtime pcr system is a powerful platform for labs requiring superior performance and maximum dye versatility. Every consumable run on a thermo fisher scientific realtime qpcr instrument requires the creation of one or more experiment files that store the associated data. Prior to downloading any software to a computer used for instrument controloperation, please. Applied biosystems 7500 realtime pcr system, desktop. How to label technical replicates in life technologies realtime pcr software. The steponeplus realtime pcr system can be setup in a variety of configurations and comes ready to use, out of the box, with intuitive data analysis and instrument control software. These factorycalibrated systems make it easy to step up to highperformance qpcr.
Applied biosystems quantstudio 6 and 7 pro realtime pcr systems are able to detect differences in target quantity as small as 1. Applied biosystems stepone and steponeplus realtime pcr. A tutorial, using example experiment data provided with the applied biosystems stepone realtime pcr software stepone software. Faster ramp rates enable rapid results without compromising extension times or assay quality. The applied biosystems steponeplus is a realtime pcr system designed to meet your needs.
The applied biosystems 7500 and 7500 fast realtime pcr systems are covered by us patent no. Detection of viral rna not only aids in the diagnosis of illness but also provides. Applied biosystems dna extract all reagents kit and used directly in qpcr by adding applied biosystems taqman gtxpress master mix. Applied biosystems stepone realtime pcr system, laptop. Monitor networked stepone and steponeplus instruments. Applied biosystems 7500 realtime pcr system thermo.
This remarkably simple realtime pcr system is designed with an lcd touchscreen and a userfriendly interface that brings the power of genetic studies to researchers new to realtime pcr fig 1. The hrm software is available on the 7500 fast realtime pcr system, which delivers precise results in a standard 96well. Applied biosystems 7500 software setup for the quantifast. The applied biosystems 7500 fast realtime pcr system offers maximum performance in the minimum time. With our applied biosystems realtime pcr platforms, you get true value with excellent performance, reliability, and worldclass support. The intuitive software and protocol wizards help guide new users through their realtime pcr experiments. Set up, run, and analyze experiments using the stepone and steponeplus systems.
Designed for compatibility with applied biosystems taqman assays and the flexibility of unlimited realtime chemistry choices, we make it easy for you to get started with your experiments. Which version of the applied biosystems 7500 realtime pcr system software can be used to run the investigator. Fully optimized for fast cycling, the 7500 fast delivers highquality results in as little as 30 minutes. Applied biosystems 75007500 fast thermo fisher scientific. The applied biosystems high resolution melting hrm software is the easiest to use melting analysis software enabling realtime pcr melt curve assays to be used more accurately for mutation scanning and genotyping. The applied biosystems taqpath covid19 combo kit is a fast, highly sensitive multiplex diagnostic solution that contains both the assays and controls needed for the realtime pcr detection of rna from the sarscov2 virus. Taqpath covid19 multiplex diagnostic solution thermo. Flow cytometry experience better data, faster speed, and simplicity with the invitrogen attune acoustic focusing cytometer.
Applied biosystems stepone realtime pcr software help explains how to use the stepone software to. Paired up with the software the steponeplus is capable of collecting melting curve data for high resolution melt also known as hrm. The allelic discrimination plot created by the desktop software figure. To verify your client connection, you need a valid user id. Perform standard pcr in less than 2 hours and fast pcr reactions in less than 40 minutes intuitive, flexible software and wizards guide new users through. The stepone realtime pcr system is a 48well, lowthroughput realtime pcr instrument perfect for both firsttime and experienced users. Applied biosystems stepone realtime pcr system getting started guide for standard curve experiments 4376784 4377736 applied biosystems stepone realtime pcr system installation, maintenance, and networking guide 4376782 4377798 applied biosystems stepone realtime pcr system installation quick reference card 4376783 4377792.
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